built-in module in graphpad prism v. 5.0 software Search Results


fluorescence intensity fi signal  (BMG Labtech)
99
BMG Labtech fluorescence intensity fi signal
Assessment of potency and cell permeability of the selected compounds. (A) Inhibition of PARK7 at 0.25 and 1 μM final concentration of 5 compounds designed from structural modification and top 10 compounds from HTS, determined using the PARK7 FP assay. (B) Fluorescent probe labeling of PARK7 remaining activity after inhibitor treatment to investigate cellular engagement. HEK293T cells were treated with the indicated compounds for 24 h. After cell lysis and incubation with the fluorescent PARK7 probe JYQ-92 for 1 h, the samples were analyzed by SDS-PAGE, <t>fluorescence</t> scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control. (C) Target engagement of compounds 84 , 336 , and 340 in HEK293T cells in a dose–response manner. HEK293T cells were incubated with the compounds at the indicated final concentrations for 24 h, prior to cell lysis and incubation with PARK7 probe JYQ-92 . The samples were analyzed by SDS-PAGE, fluorescence scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control.
Fluorescence Intensity Fi Signal, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/built-in+module+in+graphpad+prism+v%2E+5%2E0+software/PHERAstar+FSX/pmc11129182-368-1-9
Average 99 stars, based on 1 article reviews
fluorescence intensity fi signal - by Bioz Stars, 2026-09
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cell repellent plates  (Greiner Bio)
93
Greiner Bio cell repellent plates
Assessment of potency and cell permeability of the selected compounds. (A) Inhibition of PARK7 at 0.25 and 1 μM final concentration of 5 compounds designed from structural modification and top 10 compounds from HTS, determined using the PARK7 FP assay. (B) Fluorescent probe labeling of PARK7 remaining activity after inhibitor treatment to investigate cellular engagement. HEK293T cells were treated with the indicated compounds for 24 h. After cell lysis and incubation with the fluorescent PARK7 probe JYQ-92 for 1 h, the samples were analyzed by SDS-PAGE, <t>fluorescence</t> scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control. (C) Target engagement of compounds 84 , 336 , and 340 in HEK293T cells in a dose–response manner. HEK293T cells were incubated with the compounds at the indicated final concentrations for 24 h, prior to cell lysis and incubation with PARK7 probe JYQ-92 . The samples were analyzed by SDS-PAGE, fluorescence scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control.
Cell Repellent Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/built-in+module+in+graphpad+prism+v%2E+5%2E0+software/C1536W+Ultra+Low+Base+Screenstar+Plate+Co/pm38218316-66-216-219
Average 93 stars, based on 1 article reviews
cell repellent plates - by Bioz Stars, 2026-09
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t75 cell repellent surface culture flask  (Greiner Bio)
94
Greiner Bio t75 cell repellent surface culture flask
Assessment of potency and cell permeability of the selected compounds. (A) Inhibition of PARK7 at 0.25 and 1 μM final concentration of 5 compounds designed from structural modification and top 10 compounds from HTS, determined using the PARK7 FP assay. (B) Fluorescent probe labeling of PARK7 remaining activity after inhibitor treatment to investigate cellular engagement. HEK293T cells were treated with the indicated compounds for 24 h. After cell lysis and incubation with the fluorescent PARK7 probe JYQ-92 for 1 h, the samples were analyzed by SDS-PAGE, <t>fluorescence</t> scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control. (C) Target engagement of compounds 84 , 336 , and 340 in HEK293T cells in a dose–response manner. HEK293T cells were incubated with the compounds at the indicated final concentrations for 24 h, prior to cell lysis and incubation with PARK7 probe JYQ-92 . The samples were analyzed by SDS-PAGE, fluorescence scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control.
T75 Cell Repellent Surface Culture Flask, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/built-in+module+in+graphpad+prism+v%2E+5%2E0+software/Cell+Culture+Flask+550+Ml+175+Cm%C2%B2+Ps/pm38218316-66-55-62
Average 94 stars, based on 1 article reviews
t75 cell repellent surface culture flask - by Bioz Stars, 2026-09
94/100 stars
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t175 cell repellent surface culture flask  (Greiner Bio)
94
Greiner Bio t175 cell repellent surface culture flask
Assessment of potency and cell permeability of the selected compounds. (A) Inhibition of PARK7 at 0.25 and 1 μM final concentration of 5 compounds designed from structural modification and top 10 compounds from HTS, determined using the PARK7 FP assay. (B) Fluorescent probe labeling of PARK7 remaining activity after inhibitor treatment to investigate cellular engagement. HEK293T cells were treated with the indicated compounds for 24 h. After cell lysis and incubation with the fluorescent PARK7 probe JYQ-92 for 1 h, the samples were analyzed by SDS-PAGE, <t>fluorescence</t> scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control. (C) Target engagement of compounds 84 , 336 , and 340 in HEK293T cells in a dose–response manner. HEK293T cells were incubated with the compounds at the indicated final concentrations for 24 h, prior to cell lysis and incubation with PARK7 probe JYQ-92 . The samples were analyzed by SDS-PAGE, fluorescence scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control.
T175 Cell Repellent Surface Culture Flask, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/built-in+module+in+graphpad+prism+v%2E+5%2E0+software/Cell+Culture+Flask+250+Ml+75+Cm%C2%B2+Ps/pm38218316-66-44-51
Average 94 stars, based on 1 article reviews
t175 cell repellent surface culture flask - by Bioz Stars, 2026-09
94/100 stars
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Image Search Results


Assessment of potency and cell permeability of the selected compounds. (A) Inhibition of PARK7 at 0.25 and 1 μM final concentration of 5 compounds designed from structural modification and top 10 compounds from HTS, determined using the PARK7 FP assay. (B) Fluorescent probe labeling of PARK7 remaining activity after inhibitor treatment to investigate cellular engagement. HEK293T cells were treated with the indicated compounds for 24 h. After cell lysis and incubation with the fluorescent PARK7 probe JYQ-92 for 1 h, the samples were analyzed by SDS-PAGE, fluorescence scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control. (C) Target engagement of compounds 84 , 336 , and 340 in HEK293T cells in a dose–response manner. HEK293T cells were incubated with the compounds at the indicated final concentrations for 24 h, prior to cell lysis and incubation with PARK7 probe JYQ-92 . The samples were analyzed by SDS-PAGE, fluorescence scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control.

Journal: Journal of Medicinal Chemistry

Article Title: Development of Inhibitors, Probes, and PROTAC Provides a Complete Toolbox to Study PARK7 in the Living Cell

doi: 10.1021/acs.jmedchem.3c02410

Figure Lengend Snippet: Assessment of potency and cell permeability of the selected compounds. (A) Inhibition of PARK7 at 0.25 and 1 μM final concentration of 5 compounds designed from structural modification and top 10 compounds from HTS, determined using the PARK7 FP assay. (B) Fluorescent probe labeling of PARK7 remaining activity after inhibitor treatment to investigate cellular engagement. HEK293T cells were treated with the indicated compounds for 24 h. After cell lysis and incubation with the fluorescent PARK7 probe JYQ-92 for 1 h, the samples were analyzed by SDS-PAGE, fluorescence scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control. (C) Target engagement of compounds 84 , 336 , and 340 in HEK293T cells in a dose–response manner. HEK293T cells were incubated with the compounds at the indicated final concentrations for 24 h, prior to cell lysis and incubation with PARK7 probe JYQ-92 . The samples were analyzed by SDS-PAGE, fluorescence scanning, and immunoblot against PARK7 and β-actin. β-Actin was used as a loading control.

Article Snippet: The fluorescence intensity (FI) signal was monitored on a BMG Labtech PHERAstar plate reader (λ ex/em 350/450 nm) for 1 h. All samples were normalized to the positive and negative controls and plotted against the inhibitor concentrations (in μM) using the built-in equation “[inhibitor] vs response–variable slope (four parameters), least-squares fit” with constraints “Bottom = 0” and “Top = 100” in GraphPad Prism 9.0.1 software to obtain the IC 50 values.

Techniques: Permeability, Inhibition, Concentration Assay, Modification, FP Assay, Labeling, Activity Assay, Lysis, Incubation, SDS Page, Fluorescence, Western Blot, Control, Drug discovery

(A) Chemical structures of activity-based probes JYQ-191 , JYQ-192 , JYQ-196 , and JYQ-197 . (B) Labeling of purified recombinant human PARK7 with the four probes. Recombinant human PARK7 was incubated with indicated concentrations of the probes for 1 h followed by SDS-PAGE, fluorescence scanning, and coomassie staining. (C) Fluorescence labeling of PARK7 activity in HEK293T and A549 cells with JYQ-196 . HEK293T and A549 cells were incubated with indicated final concentration of JYQ-196 for 4 h, followed by cell lysis, SDS-PAGE, fluorescence scanning, and immunoblotting against PARK7 and β-actin. β-Actin was used as a loading control.

Journal: Journal of Medicinal Chemistry

Article Title: Development of Inhibitors, Probes, and PROTAC Provides a Complete Toolbox to Study PARK7 in the Living Cell

doi: 10.1021/acs.jmedchem.3c02410

Figure Lengend Snippet: (A) Chemical structures of activity-based probes JYQ-191 , JYQ-192 , JYQ-196 , and JYQ-197 . (B) Labeling of purified recombinant human PARK7 with the four probes. Recombinant human PARK7 was incubated with indicated concentrations of the probes for 1 h followed by SDS-PAGE, fluorescence scanning, and coomassie staining. (C) Fluorescence labeling of PARK7 activity in HEK293T and A549 cells with JYQ-196 . HEK293T and A549 cells were incubated with indicated final concentration of JYQ-196 for 4 h, followed by cell lysis, SDS-PAGE, fluorescence scanning, and immunoblotting against PARK7 and β-actin. β-Actin was used as a loading control.

Article Snippet: The fluorescence intensity (FI) signal was monitored on a BMG Labtech PHERAstar plate reader (λ ex/em 350/450 nm) for 1 h. All samples were normalized to the positive and negative controls and plotted against the inhibitor concentrations (in μM) using the built-in equation “[inhibitor] vs response–variable slope (four parameters), least-squares fit” with constraints “Bottom = 0” and “Top = 100” in GraphPad Prism 9.0.1 software to obtain the IC 50 values.

Techniques: Activity Assay, Labeling, Purification, Recombinant, Incubation, SDS Page, Fluorescence, Staining, Concentration Assay, Lysis, Western Blot, Control